Chemiluminescence immunoassay is currently recognized as an advanced label immunoassay technology in the world. Enhanced chemiluminescence immunoassay technology has high accuracy and specificity. Its sensitivity can reach 10 mol/L -12 (RIA is 10mol/L). It can detect substances that cannot be detected by methods such as radioimmunoassay and enzyme-linked immunoassay are of great significance to the early diagnosis of diseases. In addition, chemiluminescence immunoassay test has the advantages of stable results, small errors, good safety, long trial periods, etc.
Chemiluminescence immunoassay (CLIA) is an assay that combine chemiluminescence technique with immunochemical reactions. Similar with other labeled immunoassays (RIA, FIA, ELISA), CLIA utilize chemical probes which could generate light emission through chemical reaction to label the antibody.
For chemiluminescence it is light produced by a chemical reaction. This chemiluminescent substance can be excited by an oxidation reaction forming an intermediate. It is when this immediate return to a stable ground state happens, that a photon is released and this is detected by the luminescent signal instrument.
There are benefits of using chemiluminescence rather other types of immunoassay, including using fluorescence detection or a light absorption method, such as the fact that this technique is ultra-sensitive and can detect small amounts of the biological molecule. In addition to this, it has a wider dynamic range, as well as having a linear relationship between luminous intensity and the concentration of the measured substance. These chemiluminescent reactions can also be enhanced with an enhancer, which acts as an enzyme protector and allows the reaction to occur for a longer period of time without a large reduction in the light output. The enhanced reaction gives an intense light emission for a prolonged period of time, as well as the substrate only needing to be added minutes before detection.
